Introduction to ELEM's CRISPR Services

ELEM stands at the forefront of CRISPR gene editing technology, delivering comprehensive and highly optimized solutions tailored to meet the needs of researchers worldwide. With a proven track record and a commitment to excellence, ELEM’s services are designed to drive scientific innovation and accelerate discovery.

Our Comprehensive CRISPR Workflow

• Design, Optimization, Editing, and Analysis: ELEM provides end-to-end support for all phases of the CRISPR workflow, ensuring precision and efficiency in every step of the process.
• Engineered Cell Lines for Functional Assays: ELEM offers high-quality engineered cell lines, enabling robust downstream functional assays for a wide range of applications.

Flexible CRISPR Editing Formats

• Knockout Pool: Rapid and cost-effective solutions for high-throughput screening.
• Knockout Clone: High-precision editing with single-cell validation for targeted research needs.

Unparalleled Expertise and Methodology

• Dedicated Team of Experts: Over 20 highly skilled CRISPR scientists bring decades of collective experience to every project, ensuring the highest standards of quality and reliability.
• Industry-Leading Gold Standard: ELEM’s robust CRISPR editing methodology sets the benchmark for accuracy, efficiency, and consistency in the industry.
• Advanced Optimization Protocols: A proprietary 350-point transfection optimization procedure ensures maximum editing efficiency and reproducibility.

Proven Track Record of Success

• Extensive Editing Experience: Achieved over 12,500 individual sequence-verified edits within just two years, demonstrating ELEM’s capacity to handle projects of any scale and complexity.
• Customizable Solutions: ELEM can seamlessly work with client-provided custom cell lines or leverage its library of 380 optimized cell lines, offering unparalleled flexibility to meet diverse research requirements.

ELEM Knockout Cell Pool and Knockout Cell Clone Services

ELEM leverages its industry-leading expertise and advanced CRISPR editing methodologies to provide superior knockout cell solutions that cater to a variety of research needs. With unmatched efficiency, precision, and cost-effectiveness, ELEM’s services empower researchers to achieve their experimental goals with confidence and speed.

Knockout Cell Pool

Definition and Characteristics

• A CRISPR-edited cell population containing a mix of edited and unedited cells.
• Provides affordable access to highly edited cell pools.
• Rapid turnaround time, with delivery in as few as two to three weeks.
• ELEM manages the complex transfection process, ensuring high-quality results.
• Enables researchers to either perform clonal isolation or proceed directly to validation and functional assays.

Advantages of ELEM’s Knockout Cell Pools

• Access to ELEM’s proprietary high-throughput platform:Ensures consistent and robust editing results.
• High Editing Efficiency:Achieves knockout efficiencies averaging 85%, minimizing the need for additional optimization.
• Streamlined Workflow:Skip the lengthy cloning process and move directly to validation and assays.
• First-Pass Proof of Principle:Ideal for early-stage experiments before investing in clonal generation.
• Efficient Screening:Reduces the need to screen large numbers of clones by up to 90%.
• Minimized Off-Target Effects:Uses ribonucleoprotein (RNP) complexes to ensure precise editing with minimal off-target activity.
• Validated Results:Edited regions are confirmed via Sanger sequencing and ICE analysis for reliability.
• Cost-Effective:Costs less than 25% of the price of generating clones and includes two vials of wild-type cells valued at $500.
• Guaranteed Results:Guaranteed knockout efficiencies with a minimum of 70% protein reduction or 100% money-back guarantee.
• Unique Offering:A specialized format not available from other suppliers.

Knockout Cell Clone

Definition and Characteristics

• A monoclonal population of homozygous cells with identical knockout-inducing mutations.
• Provides 100% CRISPR-edited cell clones with precise and reliable results.
• Delivery in as few as eight to nine weeks for rapid project progression.
• ELEM handles the entire CRISPR editing workflow, from design to final delivery.
• Researchers can confidently proceed directly to validation or functional assays.

Advantages of ELEM’s Knockout Cell Clones

• Access to ELEM’s high-throughput platform: Ensures unparalleled consistency and quality.
• 100% Editing Efficiency: Delivers monoclonal populations with verified homozygous knockouts.
• Advanced Optimization: Utilizes a 150-point transfection optimization process to achieve editing success even in hard-to-transfect cell lines.
• Sequence-Verified Results: Provides 100% sequence-verified knockout of the target gene.
• Single-Cell Validation: Automated imaging confirms single-cell-derived clones for accuracy.
• Minimized Off-Target Effects: Utilizes RNP complexes to enhance cell viability and precision.
• Preserved Biological Integrity: Avoids enrichment techniques, maintaining the natural characteristics of cells.
• Rapid Turnaround: Delivered in as few as nine weeks, significantly faster than DIY approaches.
• Cost-Effective: Up to 50% lower cost compared to clones offered by competitors.
• Guaranteed Results: Guaranteed 100% protein knockout or 100% money-back guarantee.

Why Choose ELEM?

ELEM’s knockout cell solutions are designed to simplify your research, reduce costs, and accelerate timelines without compromising on quality or accuracy. With a proven track record, cutting-edge technology, and an unwavering commitment to customer satisfaction, ELEM is your trusted partner in CRISPR gene editing.